27 juin 2022 · Five hundred microliters of tissue homogenate were mixed with 400 μL of pre-warmed KH buffer. Then, 100 μL of KH buffer (blank) or 1 mg/mL EntF peptide solution (test) were added to the diluted homogenate and incubated at 37 °C. At 0, 5, 10, 30, 60, 120, and 180 min, 100 μL aliquots were taken and immediately mixed with 100 μL of 1% V/V.